0800 GMT March 30, 2020
Dr. Hossein Hassanpour, the head of the institute, added that the aim of this study is to evaluate the specificity and sensitivity of the real-time quantitative PCR method for gender testing in week nine of pregnancy.
Blood samples are collected from pregnant women prior to amniocentesis and DNA is extracted from maternal plasma.
DNA samples were subjected to real-time quantitative PCR amplification of SRY (as a fetus-specific marker) and beta-globin (as a marker for total plasma DNA) genes.
The beta-globin gene sequence is detected in all samples.
Amplification of fetal DNA from maternal plasma is a promising method for fetal sex determination in early pregnancy.
A real-time polymerase chain reaction is a laboratory technique of molecular biology based on the PCR, which is used to amplify and simultaneously detect or quantify a targeted DNA molecule.