News ID: 117552
Published: 0349 GMT May 08, 2015

Non-invasive prenatal gender testing using real-time PCR

Non-invasive prenatal gender testing  using real-time PCR

Researchers at the Research Institute of Animal Embryo Technology, affiliated to Shahrekord University, have conducted non-invasive prenatal gender testing using real-time polymerase chain reaction (PCR), an official said.

Dr. Hossein Hassanpour, the head of the institute, added that the aim of this study is to evaluate the specificity and sensitivity of the real-time quantitative PCR method for gender testing in week nine of pregnancy.

Blood samples are collected from pregnant women prior to amniocentesis and DNA is extracted from maternal plasma.

DNA samples were subjected to real-time quantitative PCR amplification of SRY (as a fetus-specific marker) and beta-globin (as a marker for total plasma DNA) genes.

The beta-globin gene sequence is detected in all samples.

Amplification of fetal DNA from maternal plasma is a promising method for fetal sex determination in early pregnancy.

A real-time polymerase chain reaction is a laboratory technique of molecular biology based on the PCR, which is used to amplify and simultaneously detect or quantify a targeted DNA molecule.



Resource: Iran Daily
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