0331 GMT August 16, 2018
While DNA nanowires have been in development for some time, the method developed at KTH Royal Institute of Technology and Stockholm University produces a unique three-dimensional biosensor for better effectiveness than flat, two-dimensional sensors, according to sciencedaily.com.
KTH Professor Wouter van der Wijngaart said, "Our geometry makes it much easier to measure several biomolecules simultaneously, and is also 100 times more sensitive.
"This is the first out-of-plane metallic nanowire formation based on stretching of DNA through a porous membrane.”
The DNA nanowires, treated with gold to make them conductive, are created only in the presence of specific biomarker molecules in the patient sample and transmit evidence of their presence, even when such molecules are low in concentration.
The conductive wires short-circuit both sides of the membrane, which makes them easy to detect.
To make the wires, the team first captured molecules on the surface of a porous membrane, which were designed to only bind with specific biomarker molecules in the sample.
Such molecular binding events then trigger the formation of long DNA wires that were drawn through the pores by vacuum drying.
Van der Wijngaart said, “Then the membrane is treated with a solution of nanometer sized gold particles, which can only bind to DNA molecules in a certain sequence.”
The researchers published their results in Microsystems and Nanoengineering (Nature Publishing Group).
In addition to van der Wijngaart and Stockholm University Professor Mats Nilsson, the authors include Maoxiang Guo (KTH) and Iván Hernández-Neuta and Narayanan Madaboosi (Stockholm University).
The research work was funded in part by the China Scholarship Council and the Swedish Research Council.