Opposition leaders have vowed to hold demonstrations until parliament is dissolved and snap elections are held.
Rallies broke out in the capital Tbilisi on Thursday after a Russian lawmaker addressed parliament from the speaker's seat, Presstv Reported.
Police fired rubber bullets and tear gas at crowds, leaving at least 160 demonstrators and 80 officers injured according to the health ministry.
The use of force in the pro-Western ex-Soviet country has sparked an international outcry.
The Kremlin condemned the protests as "Russophobic provocation".
The presence of Russian MP Sergei Gavrilov in parliament brought a backlash in Georgia, which in 2008 fought and lost a brief but bloody war with Moscow over the breakaway regions of Abkhazia and South Ossetia.
With parliamentary polls set for next year, opposition groups are also seeking to capitalize on discontent with the ruling Georgian Dream party and its oligarch leader Bidzina Ivanishvili over the failure to kick-start a stagnant economy.
"Ivanishvili's regime must cede power," former president Mikheil Saakashvili said in a video address to supporters Friday.
The United Opposition alliance has demanded snap parliamentary polls and the resignation of the interior minister.
"Peaceful mass protest will continue until these demands are met," its leader Grigol Vashadze told AFP, adding that a fresh rally would start at 7 p.m. (GMT 1500) on Friday.