Seoul and Tokyo have been embroiled in the trade and diplomatic spat since Tokyo tightened export controls in early July on three chemicals essential to making memory chips and high-spec displays, key products of South Korean companies such as Samsung and LG, Presstv Reported.
The restrictions follow a series of South Korean court rulings ordering Japanese firms to pay for forced labor during World War II.
The ongoing dispute has also seen the two neighbors remove each other from their "white lists" of trusted trading partners and prompted South Korea not to renew a military intelligence sharing pact.
"Japan's export curbs on three items are driven by political motivations linked to a Supreme Court ruling over the issue of forced labor," said South Korean Trade Minister Yoo Myung-hee at a press conference.
"Targeting South Korea is... in violation of WTO's principles banning discriminatory practice."
With South Korea's role as a main supplier for memory chips and displays, she said, the curbs have caused "significant uncertainty" in the global economy.
Yoo said South Korea will request bilateral consultation at the WTO as a first step to resolve the issue.
Tokyo says the move was made necessary by a "loss of trust" in relations with Seoul, but also accuses South Korea of improperly handling exports of sensitive materials from Japan.
But Seoul maintains it is a retaliatory move in response to historical disputes.