News ID: 262463
Published: 0906 GMT December 04, 2019

China gene-edited baby experiment 'may have created unintended mutations', scientists say

China gene-edited baby experiment 'may have created unintended mutations', scientists say
MARK SCHIEFELBEIN/AP

This is a microplate containing embryos that have been injected with Cas9 protein and PCSK9 sgRNA in a laboratory in Shenzhen, China.

The gene-editing performed on Chinese twins to immunize them against HIV may have failed and created unintended mutations, scientists said after the original research was made public for the first time.

Excerpts from the manuscript were released by the MIT Technology Review to show how Chinese biophysicist He Jiankui ignored ethical and scientific norms in creating the twins Lula and Nana, whose birth in late 2018 sent shockwaves through the scientific world, AFP reported.

He made expansive claims of a medical breakthrough that could “control the HIV epidemic”, but it was not clear whether it had even been successful in its intended purpose — immunizing the babies against the virus — because the team did not in fact reproduce the gene mutation that confers this resistance.

A small percentage of people are born with immunity because of a mutation in a gene called CCR5 and it was this gene that He had claimed to have targeted using a powerful editing tool known as CRISPR which has revolutionized the field since 2012.

Fyodor Urnov, a genome-editing scientist at the University of California, Berkeley told the MIT Technology Review, “The claim they have reproduced the prevalent CCR5 variant is a blatant misrepresentation of the actual data and can only be described by one term: A deliberate falsehood.

“The study shows that the research team instead failed to reproduce the prevalent CCR5 variant.”

While the team targeted the right gene, they did not replicate the “Delta 32” variation required, instead creating novel edits whose effects are not clear.

Moreover, CRISPR remains an imperfect tool because it can lead to unwanted or “off-target” edits, making its use in humans hugely controversial. Here, the researchers claimed to have searched for such effects in the early-stage embryos and found just one — however it would be impossible to carry out a comprehensive search without inspecting each of the embryo’s cells, and thus destroying it.

The parents’ lack of access to any kind of fertility treatment might have motivated them to take part in the experiment despite the huge risks to their children, Jeanne O’Brien, a reproductive endocrinologist at Shady Grove Fertility told the MIT Technology Review.

The father was HIV positive, which carries a significant social stigma in China and makes it almost impossible to have access to fertility treatment, even though a well-established technique known as “sperm washing” prevents the infection being passed to unborn children.

The authors also appeared to have taken steps to make it hard to find the family, like leaving the names of the fertility doctors off the paper, and including a false date of birth. He claimed November 2018 while multiple reports have indicated it was October 2018.

 

 

 

 

 

 

   
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